Rna fish
RNA FISH USING FLUOROPHORE-LABELLED OLIGONUCLEOTIDES (20-mer/Stellaris) Solutions and material Coverslips (no. 1.5 for best imaging results) 1X PBS pH 7.4 20X PBS pH 7.4 (RNAse-free) DEPC-water 36.5-38% formaldehyde (Sigma F8775, for molecular biology, contains 10-15% methanol) 70% EtOH (dilute 100% EtOH with DEPC-water) Deionized formamide (RNAse-free, store at -20ºC) Microsc