MICRONAUT-S MRSA / GP Microtitration plates for the automated or manual susceptibility testing of multi-resistent Staphylococci, Enterococci and Pneumococci
Principle, shelf-life and storage
The susceptibility testing is based on the rehydration of antibiotics by adding a standar- dized bacteria suspension (Mueller-Hinton II broth). The result is measured photometri- cally after 18-24 hours incubation at 35-37°C. Results are going to be measured and interpreted either with the MICRONAUT software or visually. Due to a special vacuum drying process the plates can be stored at a room temperature of 15-25°C. The MICRONAUT test plates have a shelf life of 24 months at date of production. Antibiotic configuration
The antibiotics configuration of the MICRONAUT-S MRSA / GP plate allows the specific detection of the clinically relevant single or multi-resistances of gram-positive bacteria of nosocomial infection. The susceptibility testing with highly effective reserve antibiotics like the MRSA cephalosprine Ceftaroline offers therapeutic options in case of extreme multi-resistance. Procedure
Prepare a bacteria suspension in NaCl (McFarland 0.5) Transfer in Mueller-Hinton II broth Inoculate MICRONAUT-S test plate Incubate for 18-24 hours at 35-37°C Measure photometrically and interprete with MICRONAUT software
Susceptibility testing of staphylococci
Penicillin G MIC: detection of staphylococcal penicillinases Detection of Oxacillin resistance by MIC determination with Oxacillin and Cefoxitin Detection of Oxacillin borderline resistance by detection of Cefoxitin susceptibility Detection of the induced MLSB resistence by Erythromycin/Clindamycin
combination test according to CLSI standard
Testing of highly effective antibiotics like Vancomycin, Linezolid, Tigecycline,
Daptomycin, and Ceftaroline as therapeutic options in case of extreme multi-resistance
Test interpretation according to EUCAST and CLSI standard
Susceptibility testing of enterococci
Ampicillin MIC: detection of ampicillin resistance Detection of the phenotypical glycopeptide resistance pattern of Vancomycin
resistant enterococci by determination of the MIC via Teicoplanin and Vancomycin
Differentiation between Enterococcus faecium and Enterococcus faecalis by
Detection of HLAR strains through high-level-resistance testing via Gentamicin and
Testing of highly effective antibiotics as therapeutic options in case of extreme
Test interpretation according to EUCAST and CLSI standard
Susceptibility testing of pneumococci
Penicillin G MIC: detection of PBP changes Detection of Ceftaroline resistance Detection of Erythromycin resistance Detection of Vancomycin resistance Moxifloxacin MIC: detection of group IV quinolones resistance Testing of highly effective antibiotics as therapeutic options in case of extreme
Test interpretation according to EUCAST- and CLSI standard
The MICRONAUT-S MRSA / GP plate is coated with these antibiotics
Abbreviation Antibiotic Abbreviation Antibiotic
Erythromycin/Clindamycin SYN
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MICRONAUT-S MRSA / GP 
Susceptibility testing of enterococci