萬能科技大學 99 學年度碩士班考試試題
一、單選題（每題 2 分）
( )1. Which is the C-terminal amino acid in the peptide His-Gln-Arg-Ser- Ala? a) Ala b) Gln c) Arg d) Ser e) His. ( )2. Which of the following is a correct listing of electronegativity values, from low to high? a) C, H, O, N, F b) N, H, O, C, F c) H, C, N, O, F d)
( )3. Which of the following pH values is the closest to the pI for an amino acid with a pK1=2.2, pK2=10.0 and pKR=4.4. a) 3.3 b) 6.1 c) 7.2 d) 8.3
( )4. What is high catalytic activity of an enzyme suggested by ? a) a low specific activity b) a low maximum velocity c) a low turnover number d)
a low Michaelis constant e)none of above.
( )5. If the y-intercept of a Lineweaver-Burk plot = 1.91 (sec/millimole) and the slope = 75.3 L/sec, Vmax equals: a) 0.0254 millimolar (mM). b) 0.523
millimolar (mM). c) 5.23 millimolar (mM). d) 39.4 millimolar (mM). e) 75.3 millimolar (mM).
( )6. In the Bohr effect the binding of oxygen to hemoglobin. a) is increased by the presence of Na+ b) is increased by the presence of H+ and CO2 c)
is decreased by the presence of H+ and CO2 d) is unchanged e) none of above.
( )7. A noncompetitive inhibitor will not change a) kcat. b) Km. c) kcat/Km d) Vmax. e) Ki. ( )8. Which of the following four fatty acids has the lowest melting point? a) CH3CH2CH2CH2CH2COOH b)
CH3CH2CH2CH2CH2CH2CH2CH2CH2COOH c) CH3CH2CH2CH2CH2CH2CH2COOH d) CH3CH=CHCH2CH2COOH.
( )9. Glycogen is a) polysaccharide storage polymer found in plants b) a linear polysaccharide c) a highly branched polysaccharide found in
animals d) a synthetic sugar substitute e) none of the above.
( )10. Pyruvate in humans cannot be converted to a) lactate b) acetyl-CoA c) oxaloacetate d) ethanol e) none of the above. ( )11. What are the three primary metabolic fates of pyruvate? a) ethanol, acetyl-CoA, glucose b) acetyl-CoA, lactate, fructose c) ethanol,
acetyl-CoA, lactate d) ethanol, creatine, glucose e) none of the above.
( )12. Which of following routes is synthesis of ATP: a) Substrate level phosphorylation b) Oxidative phosphorylation c) Photophosphorylation. d)
( )13. Lactose intolerance a) arises from inability to metabolize the disaccharide lactose b) depends on a deficiency of sucrose in the diet c) is based
on the composition of lactose, consisting glucose and fructose in glycosidic linkage d) arises because lactose is a dimer of galactose
( )14. The accumulation of citric acid will tend to a) activate glycolysis b) inhibit glycolysis c) have on effect on glycolysis d) reverse
( )15. The primary function of the pentose phosphate pathway is a) to synthesize NAD+ and pentose phosphates b) to synthesize NADPH and
pentose phosphates c) to produce NADH d) to convert pentose phosphates to metabolic intermediates for oxidative phosphorylation.
( )16. A palindrome is a) a DNA sequence that contains only one kind of base b) a DNA sequence that contains only two kinds of bases c) a
sequence that reads the same from left to right or from right to left d) none of the above
( )17. A plasmid is a) a virus that infects bacteria. b) a piece of DNA derived from two or more sources. c) an artificially created cytoplasm. d)
a small circular DNA that is not part of a bacterial chromosome
( )18. Which of the following are methods by which bacteria can be induced to take up recombinant DNA molecules? a) Heat shock. b) DNA “guns”
which spray the DNA at very high speeds. c) Electroporation d) Phage or virus infection. e) All of these are used to transform bacteria.
( )19. Cells that contain a “blue/white screening” plasmid that has an added gene are recognized by the method: a) Ability to grow on ampicillin. b)
Inability to grow on ampicillin. c) The colonies have a blue color. d) The colonies lack a blue color. e) More than one of these choices indicates that the plasmid contains recombined DNA.
( )20. The nucleotide sequence of DNA is which level of structure? a) primary b) secondary c) tertiary d) quaternary.
( )21. Which is the dominant form of DNA found in the cell? a) A b) B c) Z d) H.
( )22. The production of RNA on a DNA template is called a) replication. b) transcription. c) translation. d) nucleation.
( )23. How do origins of replication differ in eukaryotes and prokaryotes? a) Eukaryotes have several origins, while prokaryotes typically have one.
b) Prokaryotes have several origins, while eukaryotes typically have one. c) Only prokaryotes have origins of replication. d) None of the above.
( )24. The direction of synthesis of DNA is a) from the 5' end to the 3' end on both strands. b) from the 3' end to the 5' end on both strands. c) from
the 5' end to the 3' end on one strand and from the 3' end to the 5' end on the other strand. d) none of the above.
( )25. The primer for in vivo DNA replication is: a) The 3’ hydroxyl of the preceding Okazaki fragment. b) A short piece of RNA. c) A nick made
in the DNA template. d) A primer is not always required for DNA replication. e) All of these are true.
( )26. A mRNA fragment used for cloning must first be converted to DNA by: a) reverse transcriptase. b) restriction endonuclease. c) ligase. d)
( )27. Capping of eukaryotic mRNA a) occurs at the 5' end. b) occurs at the 3' end. c) occurs at both ends. d) doesn't occur at all
( )28. Which of the following RNAs is noted for having a "cloverleaf" structure? a) mRNA b) rRNA c) tRNA d) All of the above.
( )29. Which category of RNA carries amino acids for the process of translation? a) mRNA b) rRNA c) snRNA d) tRNA
( )30. How many codons are possible in a system in which a sequence of three bases specifies a single amino acid? a) 20 b) 24 c) 27 d) 64.
( )31. During elongation, tRNA binds initially to the a) “A” site on the ribosome. b) “P” site on the ribosome. c) “E” site on the ribosome. d)
( )32. The final form of mRNA in eukaryotes has all these features, except:a) There will be a special nucleotide cap on the 5’ end of the mRNA. b)
There is usually a poly A tail on the 3’ end of the mRNA. c) The mature, active mRNA contains introns. d) Only a single protein is made from any mature mRNA molecule. e) All of these are true.
( )33. A virus life cycle that involves the incorporation of the viral DNA into the host chromosome is a) lysogeny. b) lytic. c) oncogenic. d)
( )34. The following steps are all involved in genetic recombination: 1. Screening for cells that contain the recombined gene. 2. Cutting the vector
with restriction enzyme. 3. Mixing the gene of interest with the vector. 4. Isolating the gene of interest from its original source. 5. Ligating the gene of interest and the vector together. The following sequence of these five steps would be typical: a) 1→2 → 3→4 →5 b) 2 → 3→ 5→ 1→ 4 c) 5→ 4 → 3→ 2→ 1 d) 4→ 2→3→ 5→1 e) 2 → 3 → 5 → 4 → 1.
( )35. What are the primary steps for successful PCR amplification? a) denaturation, hybridization, and DNA synthesis b) denaturation,
hybridization, and DNA digestion c) renaturation, hybridization, and DNA synthesis d) renaturation, hybridization, and DNA digestion e) none of the above.
二、解釋名詞：（12%） 1. Real-time PCR 2. Gene knockout 3. Restriction enzymes 4. Transformation
三、問答題: 1. 請以 Lineweaver-Burk 圖示說明競爭(competitive)、非競爭(noncompetitive)與不競爭(uncompetitive)抑制作用，及其 K
M、Vmax 及 K M/Vmax 有何變化?。(10%)
2. 請條列式說明 DNA 與 RNA 分子在化學結構上的差異。(8%)
Prospects of utilization of the Enzymatic Protein Hydrolysate AMIZATE® in complex therapy of patients with HIV-infection and AIDS. By Gennady A. Ermolin, Professor (Immunology), Doctor of Science (Cardiology, Immunology) HIV-infection is the sluggishly progressing pathological process caused by long presence in an organism of the human immunodeficiency virus (HIV), described by infri