Lupins and lupin products
Lupin fl our is often used in meat produc-
Directive 2003/89/EC), taking effect from
also declare ingredients such as lupins and
lupin products in the ingredients list.
In order to comply with these new legislative
of analysis available to our customers, R-Biopharm decided to incorporate the new
real-time PCR Test, SureFood®Allergen Lupin from our partner Congen, Berlin,
into its product portfolio. The test has been
use of lupin proteins is causing a rise in
available since the end of April under article
allergic reactions against lupin for many
consumers. Lupin fl our presents a hazard for peanut allergy suffers, in particular, as a
In addition, we are currently working on
the development of an ELISA test system for lupin determination. We plan to launch this new product on the market at the end of the year. About our products RIDASCREEN® Gliadin competitive (R7011)
Celiac disease is permanent gluten intole-
Gliadin competitive ELISA (R7011) so as to
keep abreast of this development and to be
in a position to offer our customers the
are reversible with a gluten-free diet.
best and most reliable method of analysis for detecting gliadin in a variety of different
foods, with chemical or enzymatic pro-cesses, the gluten molecule is denatured
RIDASCREEN® Gliadin competitive is a com-
into small fragments (peptides) of different
petitive enzyme immunoassay for quantita-
sizes. These peptides in turn may also be
of prolamins from wheat (gliadine), rye (secalin) and barley (hordein) in foodstuffs
and can be used to investigate starch, syrup
our RIDASCREEN® Gliadin test (R7001) in
and beer. The specifi city of the monoclonal
sandwich-ELISA format various scientifi c
R5 antibody is so high that it is capable
groups, in particularly Prof. Mendez’ group
in Spain, have continued research into the
with a sequence of 5 – 10 amino acids.
detection of gliadin in food samples. They
fi rst test on the market to detect gluten
has only a limited suitability for detecting
The RIDASCREEN® Gliadin test requires at
the test has been offi cially available since
least two epitopes (antibody binding sites)
to form a sandwich complex. Small peptide fragments can be produced with hydrolysis, which, due to their size, only have one epitope and it is therefore impossible for a sandwich complex to form. This means that these small fragments produced by hydro-lysis are not measured.
RIDA®QUICK Afl atoxin (R5204)
• grains (maize, wheat, barley, rye, oats,
• nuts (peanut, hazelnut, almond, Brazil
Two further application notes are available on request for ginger and nutmeg, as well as for paprika, chilli and pepper. RIDA®QUICK DON (R5904)
appears for positive samples, which is not
available since April 2007. Just as it was the
case for RIDA®QUICK Afl atoxin (R5204), the test was developed by R-Biopharm and
previous test, which are listed in the follow-
Interpretation of results is made consider-ably easier, whereby a visible test band
RIDA®QUICK DON
according to the extraction procedure approx. 1.25 mg/kg (ppm) orapprox. 0.5 mg/kg (ppm)
DON content ͧ 1.25 mg/kg (ppm)(1 g sample + 40 ml extraction buffer)
DON content ͧ 0.5 mg/kg (ppm) (1 g sample + 15 ml extraction buffer)
DON content < 1.25 mg/kg (ppm)(1 g sample + 40 ml extraction buffer)
DON content < 0.5 mg/kg (ppm) (1 g sample + 15 ml extraction buffer)
test band appears with positive test band does not appear with negative samples EU-wide maximum myotoxin levels No. 1881/2006 of 19 December 2006 sett-
mycotoxin levels as defi ned in the respec-
ing maximum levels for certain contami-
tive EU regulations included in the last issue
nants in foodstuffs as a pdf fi le in German
(R-Biopharm News I/07) was unfortunately
and English on request from R-Biopharm.
not completely up to date, we apologise for
This regulation replaces the preceding ones
You have the opportunity of obtaining the
current COMMISSION REGULATION (EC) New application notes
There are several new application notes for RIDASCREEN® Chloramphenicol (R1505) and for RIDASCREEN® Enro/Cipro (R3111) available on request:
• Chloramphenicol in serum / plasma
• Chloramphenicol in mixed feed
• Enrofl oxacin and ciprofl oxacin in honey in combination with RIDA®C18 column (R2002) If you are interested in our products, please contact your local distributor. Product Recalls from food producers – information from R-Biopharm Rhône, Scotland
Since the beginning of March, there have
However, it doesn’t just seem to be compa-
been at least 7 product recalls from food
nies’ getting into trouble for missing out
producers undertaken in the UK. This is due
to the failure of companies to include the
product, a major retailer has recently been
presence of allergens on product labels. The
fi ned for claiming an ingredient was present
when in fact no detectable levels could be
milk, nuts, soya and gluten with their pre-
consumers who are either allergic or intole-
orange juice where in fact 2 students per-
rant. Although the companies involved are
forming a school project found evidence to
doing their best to ensure that all of the
the contrary This lead to the fi rm being
affected products are removed the shelves,
charged with misleading advertising over a
these recalls are still an all too common
occurrence particularly since the regulations were introduced back in Nov 2005. Update on applying EU Feed and Food Controls – information from R-Biopharm Rhône, Scotland
carried out using internationally recognised
published an update on the progress on the
protocols or where performance criteria for
analytical methods have been established.
tion 882/2004 on offi cial controls in the UK.
requirements for competent authorities, or
have been involved in many trials over the
enforcement authorities that are responsible
years, earning them the reputation as the
for checking that businesses comply with
feed and food legislation. The regulations state that food and feed should be safe with
There has not been an update since January
of this year and this latest update provides
duction and marketing of the feed. A parti-
information on on-going work at EU level to
cular area of interest within the regulation is
the characterisation of methods of analysis
controls for ’high-risk’ feed and food pro-
which states that methods be characterised
by criteria such as reproducibility, limits of detection and recoveries amongst others. The precision values referred to should be obtained either through collaborative trials
Fairs and conferences ”Vitamins in foodstuffs and food supplements“ conference 13 June 2007 in Darmstadt
The International Association for Cereal Science and Technology (ICC) in cooperation with R-Biopharm AG is organizing a Vitamin Conference in Darmstadt on Wednesday, 13 June 2007. Besides the legislative framework conditions and the nutrition physiological status quo, the focus will also be on new, improved analytical methods. The conference is aimed towards those responsible for quality assurance and the heads of laboratory in industry and in private and offi cial laboratories.
Should you have any questions, please contact Stella Lindeke, E-mail: [email protected] or Tel. +49 (0) 61 51-81 02-636.
International Association for Cereal Science and Technology (ICC) Conference and Workshop in Glasgow, Scotland 03.09. – 05.09.2007 Representatives: R-Biopharm Rhône, Glasgow Preannouncement: “Food allergens” workshop in Austria
November 2007Two-day workshop consisting of a theoretical and a practical sessionVenue: ViennaJoint event: ICC (International Association for Cereal Science and Technology), AGES (Austrian Agency for Health and Food Safety), Ofi (Austrian Research Institute for Chemistry and Technology) and R-Biopharm AG
For further information on the workshops, please contact:Christine M. Gutschelhofer, Tel: +43 (0) 664 135 21 22, E-mail: [email protected] or Skype: c.gutschelhofer
The next R-Biopharmnews will be published during the 3rd quarter 2007 R-Biopharmnews is edited by R-Biopharm AG Landwehrstrasse 54, 64293 Darmstadt Germany Phone: +49 61 51 - 81 02-0 Fax:
D R U G T H E R A P Y 50 percent is conjugated to glucuronic acid in thekidneys.9 Thus, in patients with renal insufficiency,the plasma half-life of furosemide is prolonged be-A L A S T A I R J . J . W O O D , M . D. , Editor cause not only urinary excretion but also renal con-jugation is decreased7,8,10-14 (Table 1). The other twoloop diuretics available in the United States, bumet- DIURE
Grapefruit Juice and Medications The following information is sourced directly from the Adverse Drug Reactions section of the Therapeutic Goods Administration within the Department of Health and Aging. The information below is intended for use by health professionals. If you have any questions regarding grapefruit juice and medications seek advice For more information visit: http: